International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)


A12. Analysis of Genomic Sequence of the Distal Imprinted Region of Mouse Chromosome 7

Wahyu Purbowasito1, 2, Reiko Kato1, Chikako Suda1, Hisao Shirohzu1, Takaaki Yokomine1, Ko Ishihara1, Takashi Sado1, Hiroyuki Sasaki1.
1Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540; 2Division of Disease Genes, Institute of Genetic Information, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.

A subset of genes in mammals are imprinted so that they are expressed in a parental-origin-specific manner. Most of these imprinted genes form clusters in the genome, suggesting that imprinted genes within the same cluster may be coordinately regulated by common regulatory elements such as enhancers and insulators. Identification of precise genomic structures of the imprinted genome domains should help us to understand the molecular basis of this unique gene expression system. We have constructed approximately 1-Mb contigs of yeast artificial chromosome (YAC), bacterial artificial chromosome (BAC) and cosmid clones covering the distal imprinted domain of mouse chromosome 7, which is syntenic to human chromosome 11p15.5. Complete sequencing of 4 overlapping BACs revealed the precise locations and genomic structures of the known imprinted genes as well as other non-imprinted genes and ESTs. Furthermore, a comparison of the human and mouse sequences allowed us to identify some conserved non-coding regions, which may play a role in the regulation of genomic imprinting. Taking advantage of the overlapping cosmid clones, we are currently mapping the matrix attachment regions (MARs). The features of this imprinted region will be discussed.

 


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