International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

A15. Physical Mapping of the Region Containing The New Recessive Cataract Gene (Ir2) on Mouse Chromosome 14

Hyoungnam Lee1 Sang Dal Rhee2, Sung-Don Yang2, Masaaki Okumoto3, Chang-Woo Song2 and Sungjoo Kim Yoon1
1Research Institutes of Medical Sciences, Catholic University of Korea, Seoul 137-404, Korea
2Toxicological Research Center, KRICT, Taejon Korea
3Research Institute of Advanced Science and Technology, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka, 599-8570, Japan

The new recessive cataract mouse has been identified in CXSD mice. CXSD strain is one of the recombinant inbred strain derived from BALB/c and STS/A. We placed cataract causing lens rupture 2(lr2) locus to the region near D14Mit28 in mouse chromosome 14 by fine genetic mapping using more than 586 MSM/MS F2 mice. To define lr2 - linked region physically, we constructed an STS-content map of the region spanning D14Mit158 and D14Mit30. This region completely encompasses the critical region that is defined by D14Mit87 and D14Mit86. The YAC map was constructed using a mouse physical map WC-372 as a framework. The BAC genomic library was RPCI-22 segment 2. An STS content of each clone was determined by PCR analysis for each marker. To refine the map and increase the resolution, new STSs were generated from the sequences of YAC/BAC ends. This map spans 0.7cM from 28.3 to 29.0 cM genetically. We determined the physical order of 34 STSs that were dispersed among 20 YAC clones and 21 BAC clones. Physical distance is estimated to be more than 1.1Mb by Pulsed-field gel electrophoresis of the clone inserts, thus resulting in the average resolution of 38 Kb per ordered marker. The markers included 11 genetic, 18 clone-end, 5 gene specific EST markers. Five genes include Mmot1, Gnrh, Nfl, Nfm and Adam 7, which map to the human chromosome 8p21-22. Therefore, this map represents the region synteny to this part of the human genome. In order to identify lr2 gene, more transcripts in this region are identified and the region will be sequenced.

This work was supported by a grant Molecular Medicine Research Group Program (MM-1-1) from Korean Ministry of Science and Technology.

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