International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

A25. Physical Map of the jcpk Region on Mouse Chromosome 10

Sarah J. Price1, Lisa Stubbs2, Bryan O'Dell1 and Elizabeth C. Bryda1
1Department of Microbiology, Immunology and Molecular Genetics, Marshall University School of Medicine, Huntington,WV 25704
2Genomics Division and DOE Joint Genome Institute, Lawrence Livermore National Laboratory, Livermore, CA 94550

The jcpk gene on mouse Chromosome 10 causes a severe, early onset form of polycystic kidney disease (PKD) when inherited in an autosomal recessive manner. The jcpk gene has been mapped genetically with high resolution to a region on Chromosome 10 flanked by markers 5'340N8 and 282P18T7rpt. A physical map of this region has been constructed in a series of overlapping bacterial artificial chromosome (BAC) clones. Current efforts are focused on identifying and evaluating candidates for jcpk.

The translocation mutant, 67Gso, has been reported to cause PKD in animals homozygous for the translocated chromosome and we have previously shown non-complementation of the jcpk and 67Gso mutations. Fluorescent in situ hybridization (FISH) analysis using key BACs from the jcpk region as probes confirms that the two mutations fall within overlapping regions.

The T31 mouse radiation hybrid (RH) panel has been used to map 25 anonymous kidney expressed sequence tags (ESTs). These ESTs mapped to 15 different chromosomes, including one EST that mapped near the jcpk gene and was considered as a possible candidate for jcpk. This EST has since been eliminated for consideration based on its physical localization distal to the jcpk critical region.

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