International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

A28. Mapping of the RIKEN Mouse cDNA Clones to the Human Genome Sequences: Identification of Numbers of Novel Genes that cannot be Discovered by the Combination of Exon Predictors and Existent EST Clones

Hidenori Kiyosawa1, Shinji Kondo1, Itaru Yamanaka1, Jun Kawai1, Akira Shinagawa1, Ayako Hara1, Kazuhiro Shibata1 and Yoshihide Hayashizaki1, 2, 3
1Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC) and Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
Medical School, Tsukuba University, Tsukuba-shi, Ibaraki 305-8575, Japan,
Core Research of Evolutional Science and Technology (CREST)

In an effort to build an encyclopedia of mouse genes (total genetic dictionary), we have been constructing full-length cDNA (RIKEN cDNA clones) and sequencing them. Now that the human genome sequence draft is available, we made an attempt to map our cDNA sequences onto it. The human genes on the draft were classified into two categories: known genes and predicted genes. The prediction of the genes on the human genome was performed by the combination of exon predict programs and the EST clones available to the public. At the same time, our RIKEN clones were also mapped on the human genome with aid of the exon predict programs. The locations of these human and mouse genes on the human chromosomes are presented. A comparative analysis was carried out between the mapped mouse clones and their corresponding human genes, and the third gene category "novel gene candidate" was produced. . These genes could not be identified by any other method to date, and brought to our attention only by mapping the RIKEN clones to the human genome. These genes were of great value in order to identify disease genes by positional candidate cloning approach. The statistical features found from the comparative studies are also presented.

Using the positions of known human genes and the synteny relation between human chromosomes and mouse chromosomes, the RIKEN clones are mapped also to the mouse chromosomes. The efficiency of this method is evaluated by comparing the subset of our result on known mouse genes with locus information in public databases.


We thank Tetsuya Saito1 for his contribution to map the RIKEN clones to the human genome and the data production.

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