International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)


A9. A Putative Aminophospholipid Transporter is Linked to the p Locus on Mouse Chromosome 7

Madhu S. Dhar1, Lisa S. Webb1, Loren Hauser1, David West3 and Dabney Johnson1,2
1The University of Tennessee School Genome Science and Technology, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6480, USA;
2
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-8077;
3
Parke-Davis Laboratory for Molecular Genetics, Alameda, CA 94502

Random sequencing of a selected set of overlapping bacterial artificial chromosomes (BACs) anchored on the pink-eye (p) dilution locus on mouse chromosome 7 has identified a single transcript coding for a p-locus fat-associated ATPase (Pfatp). Sequence analysis showed that this is a member of a Class V type of the third new subfamily of ATPases, suggested to be amphipath transporters. Pfatp maps distal to p between Gabrb3 and Ube3a/Ipw, the region homologous to the Prader-Willi (PWS) and Angelman (AS) Syndromes' region on human 15q11-q13. A human homologue (about 85% homologous at the protein level) of Pfatp has also been identified. Reverse-transcriptase PCR results demonstrate that Pfatp is ubiquitously expressed in human and mouse tissues, predominantly in the testes and the white abdominal adipose tissue. Physiological data will be presented suggesting that Pfatp is a strong candidate for the body-fat phenotype associated with certain heterozygous deletions around the p locus on mouse chromosome 7.


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