International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

B33. High-throughput Genome Wide Search for Mice Imprinted Genes Using Parthenogenote and Androgenote with RIKEN Full-length Mouse cDNA Microarray Analysis.

Yosuke Mizuno1,3, Yasushi Okazaki1, Yasuhiro Tomaru1, Hidenori Kiyosawa1, Yusuke Sotomaru5, Tomohiro Kohno5, Hiroshi Amanuma2,3, Masami Muramatsu1 and Yoshihide Hayashizakib1,4.
1CREST, Japan Science and Technology Corporation (JST), Genome Exploration Research Group, Genomic Sciences Center (GSC), Genome Science Laboratory and
Molecular Cell Science Laboratory, Riken Tsukuba Life Science Center, the Institute of Physical and Chemical Research (RIKEN), Ibaraki 305-0074, Japan,
Institute of biological sciences and
Institute of Basic Medical Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibarki, 305-0006, Japan,
Depertment of Animal Science, Tokyo University of Agriculture, Tokyo, Japan

Differentially expressed genes between parthenogenote and androgenote are good candidates for the identification of imprinted genes, which are specifically expressed either from the maternal or the paternal allele. To systematically search differentially expressed genes between parthenogenote and androgenote, we used RIKEN Full-Length mouse cDNA Microarray (17K). (See the abstract of Miki, R. et. al.)

We selected about 25 genes as imprinted candidates. Known imprinted genes, such as IgfII (Insulin-like-growth factor II), Snrpn (small nuclear ribonuclear protein) and Neuronatin are included.

For some of the other genes, we have confirmed these results by RT-PCR.

We have also confirmed the imprinted expressions by analyzing SNPs, which we found in the candidate genes, in two F1 crosses between B6 and Jf1 mice.

We propose a high-throughput screening strategy to systematically search the mice imprinted genes.

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