International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)


B8. Deletion Screening of the Putative Tumor Suppressor Region of Murine Acute Myeloid Leukemia on Chromosome 2 by FISH, and Characterization of Two Murine Mutants, which Delete the Same Putative Tumor Suppressor Region

Yumiko Nitta1, Kazuko Yoshida2, Kimio Tanaka1, Kenji Kamiya1.
1Res. Inst. Radiat. Biol. Med., Hiroshima Univ.,
2 Dept. Biol. Oncol., Natl. Inst. Radiol. Sci.

Radiation-induced murine acute myeloid leukemias (AMLs) are characterized by chromosome 2 deletions. The minimal deleted region (mdr), which is heterozygously deleted in over 90% of murine AMLs, is the 1.3cM interval between the two microsatellite markers, D2Mit126 and D2Mit185. Since the instability of chromosome 2 is frequently observed not only in radiation-induced but also in chemical-induced leulkemias, a tumor suppressor gene is proposed to map within the mdr. This region is homologous to human 11p11-12, a region implicated in some human AMLs.

In order to make sure the importance of mdr for the induction of leukemia, FISH screening has been carried out for 200 AMLs of C3H/He background mice stocked in Natl. Inst. Radiol. Sci., Chiba, Japan. Microsatellite markers among the mdr were prepared for probes to detect mdr-deletion.

On the other hand, we found two chromosomally deleted mutants, Del(2)59H and Del(2)Sey3H, which deleted the mdr hemizygously in the mouse stock list of the Medical Research council, Harwell, UK. Original deletions on germ line had been induced by gamma-irradiation. We have got started characterizing the radiation susceptibility for these mutants.


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