International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

B9. SDF2L1, A Putative ER Protein, is A New Member of the Pmt/rt Superfamily

Saburo Fukuda, Masaharu Sumii, Yuji Masuda, Mamoru Takahashi, Norimichi Koike, Jun Teishima and Kenji Kamiya
Department of Developmental Biology and Oncology, Division of Molecular Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University.

We isolated a novel gene encoding 221 amino acids from a murine hepatocellular carcinoma cell line. The predicted protein is similar to stromal cell-derived factor 2 (SDF2) and we named this novel gene SDF2 like 1, SDF2L1. We also isolated the human orthologue of SDF2L1. The murine SDF2L1 (mSDF2L1) amino acid sequence is 88.2% identical and 90.9% similar to the human SDF2L1 (hSDF2L1). Analysis of each genomic structure of the murine and human SDF2L1 genes revealed that each SDF2L1 consists of three exons, and a significant similarity was seen between the promoter regions. The chromosomal location of the hSDF2L1 gene was mapped to 22q11.2. Northern blot analysis revealed that both the mSDF2L1 and hSDF2L1 genes are expressed ubiquitously, with particularly high expression in the testis. Each protein predicted from both genes has the structural features of a signal peptide at the amino(N)-terminus and an endoplasmic reticulum (ER)-retention-like motif, HDEL, at the carboxy(C)-terminus. Interestingly, SDF2L1 proteins also have a limited homology with the hydrophilic region of protein O-mannosyltransferases (Pmts) of Saccharomyces cerevisiae, the human homologue of Pmts (POMT) and Drosophila melanogaster rotated abdomen (rt). These findings suggest that SDF2L1 is a novel member of the ER resident protein family as well as a new member of the Pmt/rt superfamily. Together, the high conservation of SDF2L1 among animal species, and the ubiquitous tissue distribution suggest that SDF2L1 has a function of general importance.

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