International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

C9. Microsatellite Database for MSM/Ms and JF1/Ms, inbred strains derived from Mus musculus molossinus

Ikuo Miura1, Yoshiaki Kikkawa1, Sumiyo Takahama1, Shigeharu Wakana2, Yukiko Yamazaki3, Toshihiko Shiroishi2,4 and Hiromichi Yonekawa1.
1Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Hon-komagome, Bunkyo-ku, Tokyo 113-8613, Japan;
Mouse Functional Genomics Research Group, RIKEN Gemome Science Center, The Institute of Physical and Chemical Research, 214 Maeda-cho, Totsuka-ku, Yokohama, Kanagawa 244-0804, Japan;
Genetic Informatics Laboratory, Center for Genetic Resource Information, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan;
Mammalian Genetics Laboratory, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan

The major aim of constructing this database was to establish the status of inbred strains MSM/MS and JF1/MS, which were derived from a subspecies of Mus musculus, M. m. molossinus, as genetic resources for linkage analysis and positional cloning. Other inbred strains incorporated in our database were A/J, C57BL/6J, SM/J, SWR/J, PGN2 and 129Sv/J, which have not or poorly been incorporated in the Whitehead Institute/MIT (WI/MIT) microsatellite database. The number of microsatellite loci incorporated in our database was 1,472 in total, most of which are attached with image data detecting by electrophoresis on agarose gel. 93% of the loci showed polymorphisms between the two molossinus-derived and other six inbred strains. The average distances between two neighboring loci were calculated to be 2.5 cM.

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