International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

E2. Genome Biology of C.elegans Development

Yuji Kohara
National Institute of Genetics, Mishima 411-8540, Japan

Considering the occurrence of rounds of genome duplication during the evolution leading to vertebrates that include ourselves, genome analysis of invertebrates is essential for human genome analysis.

We have been performing expression and functional analysis of the 100-Mb genome of the nematode C.elegans. Some 19,000 genes have been predicted from the genome sequence and we identified about 10,000 cDNA species through our EST project. We have analyzed their expression patterns systematically by using of our whole mount in situ hybridization method. Thus far, hybridization with some 7000 genes has been done, and the annotation of the expression patterns (spatial and temporal) are being provided.

Based on the annotation of about 5000 genes, we have done clustering analysis of the expression patterns. The analysis provided us various clusters for embryonic expressed genes, e.g. a group of genes which show gut lineage specific expression but different start of transcription. The analysis of the regulatory regions for such lineage/stage specific expression is in progress by using of experimental and in silico approaches. Function analysis of the clustered genes are also being performed by using of RNAi and 4D microscope.

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