International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)


F9. Exploring Metabolic Pathways in 49 Tissues using RIKEN Full-Length Mouse 19K cDNA Microarray

Rika Miki1, Hidemasa Bono1, Yosuke Mizuno1,2, Koji Kadota1, Yasushiro Tomaru1, Piero Carninci1, Kazuhiro Shibata1,2, Masayoshi Itoh1,2, Jun Kawai1,2, Hideaki Konno1,2, Yumiko Tokusumi1, Yoshiyuki Ishii1,Masami Muramatsu1,2, Josephe DeRisi3, Vishy Iyer4, Michael Eisen4, Patrick O. Brown4, Yasushi Okazaki1, Yoshihide Hayashizaki1,2,5

1Genome Exploration Res Grp, GSC/Genome Science Lab, Tsukuba, RIKEN,
2
CREST, JST,
3
Univ of California,
4
Stanford Univ,
5
Med, Tsukuba Univ

The goal of genome project is not to determine the genome sequence itself but to know the mechanism of living things by analyzing how the genes are functioning. cDNA microarray technique has been developed to systematically analyze the alteration of gene expression. This technique is expected to contribute to the functional analysis in the post-sequence era. So we have constructed a systematic characterization of gene expression using the mouse full-length cDNA microarray containing 19K unique genes set (http://genome.rtc.riken.go.jp).

These set have been used to profile expression patterns of 49 adult and embryonic tissues. In this study, we focused on the genes that relate to the metabolic pathway and 1979 genes were mapped on the 80 pathways. These genes were clustered based on their expression level using the hierarchical clustering method. We found that genes on the same pathway were roughly divided into two different groups, one ubiquitously expressed and the other, tissue-specifically expressed. We have also developed a tool (ABSTRACT method) to find genes that are inversely correlated. We will report these genes and gain some insight into the mechanism of metabolic pathway.


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