International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

I5. A Translation Elongation Initiation Factor Gene Eif2s3y on the Mouse Y Chromosome is Required for Normal Spermatogonial Proliferation

Sophie Mazeyrat1, Eva Eicher2, Colin Bishop3, Paul Burgoyne1 and Michael J. Mitchell4
1Developmental Genetics, N.I.M.R., The Ridgeway, Mill Hill, London NW7 1AA, U.K.,
The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, U.S.A.,
Dept of Ob/Gyn, Baylor College of Medecine, 6550 Fannin, Houston, TX 77030, U.S.A.,
Inserm U.491, Faculte de medecine, 27 bd Jean Moulin, 13385 Marseille cedex 05, France.

The Sxrb deletion interval of the short arm of the mouse Y chromosome is necessary for the proliferation and survival of the spermatogonial germ cells in the newborn testis. This genetic function has been termed Spy (spermatogenesis on the Y), but the gene(s) responsible have not yet been identified. We and others have established a transcription map of the critical interval and to date eight transcribed genes have been identified, all of which have a closely-related homologue on the X chromosome. Five of these genes are transcribed 3 days post-partum (dpp), the time when the effects of the deletion are first evident. These genes are Smcy (a putative transcription factor), Ube1y (ubiquitin-activating enzyme E1 homologue), Uty (putative transcription repressor), Dby (a putative translation factor) and Eif2s3y (elongation initiation factor 2 subunit 3 homologue). We have established transgenic lines for Ube1y (cosmid), Dby (BAC) and Eif2s3y (BAC), and crossed these into mice carrying the Sxrb deletion. Normal spermatogonial proliferation was observed in the presence of the Eif2s3y transgene alone but not in the presence of the Ube1y or Dby transgenes, either alone or in combination. Our results show that Eif2s3y is the only gene on the mouse Y chromosome that is critical for the mitotic stages of spermatogenesis. Our identification of Eif2s3y as Spy represents the second assignment of a biological function to a gene on the mouse Y chromosome.

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