International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


Dr Patricia Baldacci
Institut Pasteur
Unité Biologie du Développement
Institut Pasteur, 25 Rue de Dr Roux
75724 Paris Cedex 15 France

Co-Authors:  Le Bras S, Cohen-Tannoudji M, Guyot V, Vandormael-Purnin S, Couailleau F, Babinet C
Institution:   Unité Biologie du Développement, Institut Pasteur

The DDK syndrome is defined as the embryonic lethality, at the morula-blastocyst stage, of F1 embryos from crosses between DDK females and males from other strains. Genetically controlled by the Om locus, it is due to a deleterious interaction between a maternal factor present in DDK oocytes and the non-DDK paternal pronucleus. Thus, the DDK syndrome constitutes a unique genetic tool to study the crucial interactions that take place between the parental genomes and the egg cytoplasm during mammalian development. We have performed an extensive analysis of the Om region by exon trapping. 150 unique sequences were identified of which 27 corresponded to genes in the databases: b-adaptin, CCT zeta2, DNA LigaseIII, Notchless, Rad51l3 and Scya1. 28 other sequences presented homologies with ESTs and genomic sequences whereas 57 did not. The pattern of expression of 37 of these markers was established by RT-PCR. Importantly, the expression of 5 sequences was detected in DDK oocytes making them candidate genes for the maternal factor. 20 sequences were expressed in testis and are candidate genes for the paternal factor. These results are an important step forward in identifying the genes responsible for the DDK syndrome.

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