International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 5 - CONSTRUCTION OF CHROMOSOME SUBSTITUTION STRAINS OF THE EXTREME BIG AND FAT MOUSE LINE Du6i

Mrs Marianna Bevova
Research Institute for the Biology of Farm Animals
Dept. Molecular Biology
Wilhelm-Stahl-Allee 2
18196 Dummerstorf Germany

Co-Authors:  Renne U and Brockmann GA
Institution:   Research Institute for the Biology of Farm Animals

The high body weight selected inbred line Du6i is twice as heavy and three times as fat as the unselected control line. Mice of line Du6i are characterized by hyperinsulinemia, hyperleptinemia, highly increased serum concentrations of IGF-1 and IGF binding proteins as well as triglycerides and cholesterol. QTLs for body composition traits as well as for serum parameters have been mapped on several different chromosomes in previous experiments of crosses between Du6i and DBA/2 (G.A. Brockmann et al. Genome Res. 2000 10:1941-1957; G.A. Brockmann et al. 2001 FASEB J. 15:978-987). Gene-gene-interaction between QTLs has been shown by statistical tests. For the final identification of underlying genes in congenic lines and for the targeted analysis of gene-gene-interaction we are constructing chromosome substitution strains (CSS) for the extreme mouse line Du6i. Every CSS has a single chromosome from the donor strain substituting for the corresponding chromosome in the host strain. We use the mouse line Du6i as donor line and the commercial inbred line DBA/2 as recipient line. CSS are established by recurrent backcrossing to DBA/2 line. The transfer of the chromosome of interest as well as the genetic background of the recipient line are controlled by genetic markers covering the entire genome. Currently we are at the fifth generation of backcross for most of the CSS. We will show the present result of our work.


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