International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 151 - DETECTION OF QTL FOR BODY WEIGHT IN MICE USING RECURRENT BACKCROSSING TO DEVELOP CONGENIC LINES

Dr Charlotte Bruley
The University of Edinburgh
Institute of Cell, Animal and Population Biology
University of Edinburgh
West Mains Road
Edinburgh EH9 3JT

Co-Authors:  Hill WG and Bunger L
Institution:   Institute of Cell, Animal & Population Biology (ICAPB), University of Edinburgh

We have a unique set of extreme selected lines of mice in our laboratory derived from over 60 to 100 generations of selection for growth, with the heaviest mouse line 5 times heavier than the smallest. They are an important resource for mapping loci associated with quantitative traits (QTL) affecting growth and identifying the relevant genes.

We aimed to simultaneously identify and prepare the ground for isolating QTL of large effect on growth by recurrent backcrossing and selection. By use of an inbred recurrent line with backcrossing and selection in single-family replicate lines, these lines will become congenic to the recurrent parent, except for QTL of large effect and closely linked regions of the genome. Such lines should be valuable for subsequent fine-scale mapping and gene cloning as by chance different QTL will be retained in different lines.

An experiment has been initiated by recurrent backcrossing and selection for body weight of the heaviest line, DUH (BW = 83g at 70d), to another inbred high body weight line EDHi (BW 70 = 47g). After 5 generations of backcrossing with selection, four lines were established which are about 5g heavier than EDHi and these were then mated inter se, with selection, to fix QTL for high body weight from DUH. Broad scale and then fine scale mapping are underway to locate QTLs for further analysis. Results on regions of the genome showing associations with body weight will be presented.


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