International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


Dr Julian Christians
ICABP, University of Edinburgh
Ashworth Laboratories
King's Buildings
West Mains Road
Edinburgh EH9 3JT

Co-Authors:  Morris KH, Ishikawa A, Oliver F, and Keightley PD
Institution:   Institute of Cell, Animal and Population Biology, University of Edinburgh  

Fine mapping of a quantitative trait locus with a major influence on murine growth

Many traits of medical or agricultural significance show continuous variation and in recent years there has been an increased focus on identifying the genomic regions that influence such traits (i.e., quantitative trait loci, or QTL).  Such work has often been successful at identifying QTL and estimating their effect and location but it has proven much more difficult to map QTL with sufficient resolution to identify the underlying genes.  However, by using an interval specific congenic strain (ISCS) to examine a QTL with a large effect on murine growth, we hope to refine the position of a QTL enough to allow the identification of a small number of candidate genes.  Previous work on an F2 cross between C57BL/6J and DBA/2J identified a growth QTL on chromosome 1 and mapped it to a ~12 cM region.  This QTL had a large effect on tail length (1.3 standard deviations between homozygotes), accounting for 21% of the variation in this trait.  The QTL also had a smaller effect on body mass (0.62 standard deviations, accounting for < 5% of the variation in the trait).  We have further refined the position of this QTL by genotyping at additional markers.  Furthermore, we have created an ISCS by introgressing the region containing the QTL from the C57BL/6J strain onto the DBA/2J background.  Preliminary work with this ISCS indicates that introgression was successful and confirms the magnitude of the effect found in the original experiment.

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