International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 130 - UK MOUSE SEQUENCING CONSORTIUM: FINDINGS FROM FINISHED SEQUENCE

Ms Victoria Cobley
UK MRC HGMP Resource Centre
Hinxton
Cambridge
CB10 1SB

Co-Authors:  3)Denny P, 5)Hummerichs H, 4)Cross S, 4)van Heyningen V, 4)Gautier P, 1)Botcherby MRM, 1)Leaves N, 1)Greystrong J, 1)Greenham L, 1)Jones S, 1)Maggott K, 1)Manjunath S, 1)Russell E, 1)Strachan G, 1)North P, 1)Boal E, 1)Hunter G, 1)Kimberley C, 1)Cave-Berry L, 2)Oliver K, 2)Simms S, 2)Gregory S, 2)Evans R, 2)Hubbard T, 2)Durbin R, 7)Birney E, 3)Cadman M, 3)McKeone R, 3)Sellick C, 3)Strivens M, 3)Mallon AM, 5)Iravani M, 4)White S, 6)Little P, 4)Jackson I, 2)Rogers J, 3)Campbell RD, 3)Brown SDM
Institutions: 1)MRC UK-HGMP Resource Centre, 2)Sanger Centre, 3)MRC UK Mouse Genome Centre and Mammalian Genetics Unit, Harwell,  4)MRC Human Genetics Unit, Western General Hospital, 5)Imperial College, 6)School of Biochemistry & Molecular Genetics, University of New South Wales, 7)EBI

The UK mouse sequencing consortium was initiated in October 1999 to underpin the UK mouse genomics effort by obtaining 50 Mb of genomic sequence targeting four regions which have been the subject of intensive investigation in the UK:

1)The WAGR-homologous region on chromosome 2

                2)The brown deletion complex on chromosome 4

                3)The Del(13)Svea36H chromosome 13 deletion

                4)Dmd-Ar region on chromosome X

The project complements ENU mutagenesis programmes underway at Harwell and HGU that are focused on the recovery and characterisation of mutations from the chromosome 2, 4 and 13 deletion regions. The project aims to discover novel genes and features in the targeted regions and to construct complete transcript maps with accurate gene models derived from bioinformatic and experimental analysis. Obtaining the finished genomic sequence of these three regions will greatly improve the efficiency of mutation scanning and the identification of genes underlying mutations of interest.  The first phase of the project required the production of maps and minimal tiling paths from the RPCI23 C57BL/6J library and was carried out by the mapping groups at HGU, Harwell and Imperial College.  As the sequencing data accumulates certain aspects of the mouse genome have become apparent and further regions of interest have been identified. Data highlighting some of the problems associated with the mouse genome and some specific areas of interest is presented.


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