International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


COMPARATIVE MAPPING OF HUMAN Chr 21 AND MOUSE Chrs 16 AND 17, AND GENE CONTENT OF TS65DN

Muriel Davisson
The Jackson Laboratory
600 Main Street
Bar Harbor Maine 04609 U.S.A.

Co-Authors: 2)Gardiner K, 1)Akeson EC, 1)Bechtel LJ, 2)Fortna A, 2)Slavov D, 1)Schmidt C
Institutions: 1)The Jackson Laboratory, 2)The Eleanor Roosevelt Institute

Human Chr 21q is conserved, centromere to telomere, in mouse, Chrs 16, 17 and 10. Ts65Dn mice are trisomic for much of the Chr 16 segment. To facilitate the construction of appropriate mouse models of Down syndrome we have mapped additional genes in mouse Chrs 16 and 17 and identified additional genes in the Ts65Dn segment. The annotated complete genomic sequence of human Chr 21 was used to identify relevant genes, followed by database searches to identify the homologous mouse genes. The mouse T-31 radiation hybrid (RH) panel, FISH, and genomic sequence analysis were used to map these genes to the mouse chromosomes. These data show that the mouse Chr 17 component is about 1.5 Mb and contains possibly as few as 21 genes. The Chr 16 breakpoint of the Ts65Dn chromosome lies between Ncam2 and App/Gabpa and the Ts65Dn chromosome contains ~60% of the human Chr 21 homologs. The mouse Chr 16 - Chr 17 evolutionary breakpoint is between human genes ZNF295 and UMODL1. The Chr 17 - Chr 10 breakpoint is between KIAA0179 and PDXK, and appears to have involved a duplication of the PDXK gene, which is present as a pseudogene, Pdxk-ps, in mouse Chr 17.


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