International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 155 - A PUTATIVE MURINE AMINOPHOSPHOLIPID TRANSLOCASE MAPS TO A QUANTITATIVE TRAIT LOCUS INFLUENCING BODY FAT IN MOUSE CHROMOSOME 7

Dr Madhu Dhar
University of Tennessee
Graduate School of Genome Science and Technology
Oak Ridge National Laboratory.
Oak Ridge
TN 37831
USA

Co-Authors:  1)Sullivan C, 2)Moustaid NM, 3)Johnson DK
Institutions: 1)University of Tennessee, Graduate School of Genome Science and Technology, 2)Department of Nutrition, University of Tennessee, 3)Life Sciences Division, Oak Ridge National Laboratory

A putative murine aminophospholipid translocase maps to a quantitative trait locus influencing body fat in mouse chromosome 7

Quantitative trait locus (QTL) analyses of obesity in mice have identified a QTL affecting body fat content and linked to the pink-eyed  (p) dilution locus on mouse chromosome 7. We have shown that a genetic locus, p-locus-associated obesity 1 (plo 1), influences body fat and maps to the same region on mouse chromosome 7 as the QTL. Sequencing of a set of overlapping bacterial artificial chromosomes (BACs) mapping to this region has identified a transcript coding for an ATPase (Pfatp).Sequence analysis proved this to be a member of the Class V of the third new subfamily of ATPases, proposed to be amphipath transporters or aminophospholipid translocases.Pfatp maps distal to p between Gabrb3 and Ube3a/Ipw, the region homologous to the Angelman (AS) Syndrome region of human 15q11-q13.  A human ortholog, PFATP is 82% identical at the protein level and maps to human chromosome 15q12.Expression analysis shows that both the murine pfatp and its human counterpart PFATP are expressed in white abdominal adipose tissue. The gene organization is similar in both organisms, with the conservation of all exon-intron junctions. Ours and other studies suggest a role for this novel ATPase in lipid/fat metabolism in mouse and human adipose tissue. Physiological data will be presented to show the expression of this gene in mouse adipose tissue in vivo and in murine 3T3-L1 adipocytes in vitro.


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