International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


EPIGENETIC REPROGRAMMING AFTER NUCLEAR TRANSFER

Kevin Eggan
Whitehead Institute for Biomedical Research
Department of Biology
Massachusetts Institute of Technology
9 Cambridge Center
Cambridge MA 02142, USA

Co-Authors: 1,2)Eggan K, 1,2)Humpherys D, 3)Akutsu H, 1)Hochedlinger K, 1)Rideout III W, 3)Yanagimachi R, 1,2)Jaenisch R
Institution: 1)Whitehead Institute for Biomedical Research, 2)Massachusetts Institute of Technology, 3)John A. Burns School of Medicine, University of Hawaii

Cloning by nuclear transfer is an inefficient process with most clones dying prior to birth and survivors often displaying growth abnormalities. In an effort to correlate gene expression with survival and fetal overgrowth, we have examined imprinted gene expression and X-chromosome inactivation in cloned mice. Although normal X-inactivation was recapitulated in cloned mouse embryos, variation in imprinted gene expression was observed in most mice cloned from embryonic stem (ES) cells, even in those derived from ES cells of the same sub-clone. Many of the cloned animals survived to adulthood despite improper expression of several imprinted genes, indicating that mammalian development may be rather tolerant to epigenetic abnormalities. Our results suggest that epigenetic processes ordinarily transpiring after fertilization may occur normally in cloned mice but that epigenetic information customarily imposed on the genome during gametogenesis may not be recovered if lost during differentiation or aging of the donor cell nucleus. Therefore, even apparently normal cloned animals may have subtle abnormalities in gene expression.


Abstracts * Officers * Bylaws * Application Form * Meeting Calendar * Contact Information * Home * Resources * News and Views * Membership

Base url http://imgs.org
Last modified: Saturday, November 3, 2012