International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


PHENOTYPIC ANALYSIS AND CHROMOSOMAL MAPPING OF MOUSE MUTANTS WITH IMMUNOLOGICAL DEFECT GENERATED BY ENU-MUTAGENESIS

Heinrich Flaswinkel
TU-München
Institut für Med. Mikrobiologie
Troger Str. 4a
München 81675, Germany

Co-Authors: 2)Rathkolb, B, 3) Faerber, C, 1) Servatius, A, 4) Soewarto, D, 5) Kremmer, E, 1)Sandholzer, N, 4) Hrabe de Angelis, M, 6)Balling, R, 2) Wolf, E, 1)Pfeffer, K
Institutions: 1) Institute of Medical Microbiology, Immunology & Hygeine, Universität München, 2)Institute of Molecular Animal Breeding, Gene Center, 3) Ingenium Pharmaceuticals, 4) Institute of Experimental Genetics, GSF Research Center for Environment and Health, 5) Institute of Molecular Immunology GSF Research Center for Environment and Health, 6) Gesellschaft für Biotechnologische Forschung

Analysis of gene function in vivo is currently mostly performed by transgenic insertion, inactivation of the respective gene by homologous recombination in embryonic stem cells and gene trapping. We have complemented this approach by isolation of mouse lines with phenotypic immunological alterations which were produced within the Munich ENU-mouse mutagenesis project.

In this phenotypic approach C3HeB/FeJ are randomly mutagenized using the potent mutagen ethyl-nitrosourea (ENU). ENU is most potent during spermatogenesis and therefore mutagenesis is performed on male mice which are subsequently crossed to female WT mice. Progeny thereof are analyzed for inherited immunological alterations using a panel of immunological parameters.

Out of more than 50 mutant lines isolated with this approach we have analysed two in more detail and mapped the corresponding mutation. Data from this analysis will be presented. <> in Microsoft Word 6.0 format with margins of 2.5cm together with all of the details requested in this form.


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