International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 194 - COMPARATIVE METHYLATION ANALYSIS OF PREDICTED CPG ISLANDS IN MOUSE AND MAN

Mrs Petra Galgoczy
Institute of Molecular Biotechnology
Department of Genome Analysis
Institute of Molecular Biotechnology
Beutenbergstr. 11a
Jena
07745
Germany

Co-Authors: Rosenthal A, Platzer M
Institutions: Institute of Molecular Biotechnology

Xq28 is one of the most gene dense regions of the human genome including more than 30 disease genes. We use comparative mapping and sequencing of 6 Mb syntenic region between Ids and Dmd of the mouse X to investigate the organization/evolution of Xq28 and as a tool for detection of novel regulatory elements.

Methylation of CpG islands in higher eukaryotic insulates sequences from the interaction with DNA binding proteins. Only unmethylated promoter regions are accessible targets for binding and interaction.

We have analyzed more than 600 kb syntenic sequences in mouse and man for the occurrences of CpG islands: In the regions ZNFP92 to PLXN6 and FLN to NEMO we detected 32 putative CpG islands in man and 24 in mouse. Only 50% of them are associated with the 5' end of known genes. We try to elucidate, whether the additional CpG islands are clues for yet unknown genes. Therefore we have examined the methylation status of 15 selected CpG islands conserved in both species using the bisufite genome sequencing technology. Heavily methylated CpG islands do not represent active promoter elements and were excluded from further analysis. In contrast, at least four CpG islands were found to be unmethylated in both species and therefore may indicate the presence of not yet identified genes. Furthermore, we present detailed comparison of methylated versus unmethylated CpG islands to improve the prediction of functional CpG islands in man and mouse


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