International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 159- IDENTIFICATION OF A NOVEL COMMON SITE OF RETROVIRAL INTEGRATION ON MOUSE CHROMOSOME 15 FROM AKXD B-CELL LEUKEMIAS

Dr. Kathryn Hentges
Baylor College of Medicine
One Baylor Plaza
Room S405
Dept. of Molecular and Human Genetics
Houston
77030
USA

Co-Authors: Justice, M.
Institutions: Baylor College of Medicine, Dept. of Molecular and Human Genetics,

A retroviral insertion site from the AKXD-27 recombinant inbred strain of mice was identified through the VISA technique (Hansen et al., Genome Research 10:237-243).  This viral insertion site was mapped to mouse chromosome 15, near the gene SREBF2. The VISA tag for this site showed homology to a human EST clone on human chromosome 22, in a region of homology with the mouse insertion site.  Further analysis of the insertion site by Southern blotting in tumors from the AKXD strains of mice demonstrated rearrangements in approximately 10% of tumors from these mice.  By comparing the human and mouse genomic sequence we discovered that there are three novel genes that surround the viral insertion site. Mouse EST clones for these three genes are found in libraries from spleen, liver, B-cells, embryo, brain, and various tumors, suggesting that they may function in lymphoid tissues and contribute to the leukemias seen in these mice.  Mouse EST clones for these three genes have been sequenced. All three genes are predicted to code for small proteins (less than 200 amino acids) with no defined domains. Northern blotting and RT-PCR were used to determine the wild-type expression pattern of these genes. The expression of the three genes in AKXD tumor tissue samples with the viral insertion will be examined.  A mouse BAC clone corresponding to this region has been isolated and submitted for sequencing.


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