International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 22 - HIGH RESOLUTION MAPPING OF THE QUANTITATIVE TRAIT LOCI RESISTANCE TO MALARIA USING ADVANCED INTERCROSS LINES OF MICE: A STEP TOWARDS CLONING THE CORRESPONDING GENES

Maria Hernandez
International Livestock Research Institute
Lab 7
Nairobi, Kenya

Co-Authors: 1&2) Hernandez Valladares M, 1) Naessens J, 1) Gibson J, 1) Musoke A, 2)Onesmo K. ole-MoiYoi, 1) Iraqi F
Institutions: 1) International Livestock Research Institute (ILRI), 2) Institute of Molecular and Cell Biology-Africa

Malaria is the most severe parasitic disease of man. Because of the current widespread distribution of drug resistant strains of Plasmodium falciparum, there is a desperate need to develop anti-malarial vaccines. Such an effort would be greatly facilitated by improvement of our understanding of the mechanisms of resistance and immunity to malaria.

Quantitative trait loci (QTL) for disease resistance may comprise many genes that facilitate host protection at any of several levels of defence. These complex traits can be easily dissected in genetically well-defined inbred mouse strains. C57Bl/6 and A/J inbred strains of mice are known to be resistant and susceptible to Plasmodium chabaudi, respectively. Recent investigations have used these mice as parent stocks to conduct genome-wide searches of QTL for resistance to malaria. Bioassay and genetic analyses of the F2 generation and back-crosses revealed strong evidence for QTLs on chromosomes 5 and 8, and weaker linkages on chromosomes 15 and 17, which were mapped to within a 20-30 cM genomic region.

Darvasi and Soller (1995) have described the use of advanced intercross lines (AILs) as an efficient tool for dissection and fine mapping of QTL. Recently, we have developed a F11 AIL population of C57Bl/6 x A/J mice. The mice were challenged with Plasmodium chabaudi chabaudi. Genetic analyses of F11 AIL mice have confirmed the previous mapped QTLs on chromosome 5, 8 and 17, within a narrower genomic region. The use of the F11 population has increased substantially the degree of resolution for malaria resistance QTL mapping and could lead to positional clone of candidate malaria resistance gene(s) in the mouse.


Abstracts * Officers * Bylaws * Application Form * Meeting Calendar * Contact Information * Home * Resources * News and Views * Membership

Base url http://imgs.org
Last modified: Saturday, November 3, 2012