International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 196 - Advanced Integrated Mice YAC Map including BAC & Corporate Analysis Framework

Heinz Himmelbauer
Max-Planck-Institute of molecular Genetics
Ihnestraße 73
D-14195
Berlin
Germany

Co-Authors: 1, 2)Schalkwyk L, 2)Cusack B, 2)Dunkel I, 2)Hopp M, 2)Kramer M, 2)Palczewski S, 2)Piefke J, 2)Scheel 2)S, Weiher M, 2) Wenske G, 2)Lehrach H,  2)Himmelbauer H
Institutions: 1)Social, Genetic and Developmental Psychiatry Research Centre, Institute of Psychiatry, 2) Max-Planck-Institute of Molecular Genetics

We have generated an advanced physical map of the mouse. Using interspersed repetitive sequence (IRS)-PCR, we generated 15000 probe fragments mainly from mouse YAC and BAC genomic clones and subsequently hybridised them against arrays containing 3D-YAC pools comprising 18 genome equivalents. The data obtained was used for map construction together with data from other sources (Hunter et al., 1996; Nusbaum et al., 1999). The final map contains 20205 markers, of which 12033 are novel and from our own data, and a total of 56093 YACs (see www.molgen.mpg.de/~rodent). The map also locates almost 2000 BACs and therefore represents an initial global mouse BAC framework map. A physical map is a prerequisite for map-based (positional) cloning of genes. In particular, the sequence of the mouse genome will remain incomplete for years to come. Thus, even with efforts proceeding rapidly to sequence the mouse genome, both public and private, the physical map that we have produced will have lasting value as a crucial instrument to proceed from phenotypes to the genes.


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