International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)

POSTER 121 - The MRC Harwell Microarray Facility: Analysing the mouse transcriptome using DNA microarrays

Dr Zuzanna Tymowska-Lalanne
Medical research Council
Harwell Microarray Facility,
Medical Research Council,
Harwell, Oxfordshire OX11 ORD, UK

Co-Authors: 1) Pritchard C, 1) Smith L, 1) Underhill P, 1) Smythe S, 1) Breeds S, 1) Abdul-Husein S, 2) Freeman T, 2) Williams D, 1) Webb S, 1) Pilicheva K, 1) Stivens M, 1) Greenfield A 1) Brown S
Institutions: 1) Harwell Microarray Facility, Medical Research Council, Harwell, 2)Microarray Group, MRC UK HGMP Resource Centre, The Genome Campus

At the MRC Mammalian Genetics Unit, Harwell, we are using cDNA microarrays to explore gene transcription in a number of biological systems, with mouse models as the common theme. We are examining the development of particular organs, for example the gonad, as well as profiling expression in complex adult structures such as the cochlear and muscle. More recently our work has begun to focus on using microarrays as another tool for the phenotypic analysis of mouse mutants, many of which have been generated by the ENU mutagenesis program at Harwell.

The MRC Harwell Microarray Facility was established in May 2000. In collaboration with the HGMP Resource Centre (Hinxton, Cambridge) we aim to promote microarray technology via the dissemination of resources and expertise. We have recently established a number of collaborations with external groups wishing to incorporate expression profiling into their projects, with particular emphasis on examining novel mouse mutants.

We are analysing microarrays from a variety of sources, including normalised cDNA libraries from particular tissues. Our central resource currently is the 15K minimal set constructed at the NIA, USA by Minoru Ko. Our facility utilises the Affymetrix 417 arrayer and the Affymetrix 428 scanner. Data are analysed using the Imagene and Genespring software packages. We are also currently developing a LIMS system to allow efficient storage and analysis of microarray data.

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