International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 199 - GENETIC, PHYSICAL AND COMPARATIVE MAP OF THE SUBTELOMERIC REGION OF MOUSE CHROMOSOME 4

Dr. Xia Li
Monell Chemical Senses Center
3500 Market Street
Philadelphia
PA 19104-3308
USA

Co-Authors: 1)Bachmanov AA, 1)Li S, 1)Chen Z, 1)Tordoff MG, 1,2)Beauchamp GK, 3,4)de Jong PJ, 4)Wu C, 4)Chen L, 4)West DB, 4)Ross DA, 4)Ohmen JD, 1) Reed DR
Institutions: 1)Monell Chemical Senses Center, 2)University of Pennsylvania, 3)Children's Hospital Oakland Research Institute, 4)Pfizer Global Research and Development

The subtelomeric region of mouse chromosome 4 harbors loci with effects on behavior, development and disease susceptibility. Regions near telomeres are more difficult to map and characterize than other areas due to the unique features of subtelomeric DNA. Therefore, we sought to characterize the area in detail by creating a comprehensive genetic, physical and comparative map. The genetic map contained 30 markers and covered a 13.3 cM distance; we then constructed a 1.2 Mb sequence-ready BAC contig, representing a 5.1 cM area, and sequenced a 246 kb mouse BAC from this contig. Using the resulting sequence, as well as ~40 kb of previously deposited genomic sequence, yielded a total of 284 kb of sequence, which contained over 20 putative genes. These putative genes were confirmed either by matching ESTs or cDNA in the public databases and/or by direct sequencing of cDNA. Comparative analysis demonstrated conserved synteny between the mouse and the human genomes (1p36.3). DNA from two strains of mice (C57BL/6ByJ and 129P3/J) was sequenced to detect SNPs. The frequency of SNPs in this region was more than three-fold higher than the genome-wide average for comparable inbred mouse strains (129/Sv and C57BL/6J). These data will facilitate genetic and comparative mapping studies and identification of a large number of novel candidate genes for the trait loci mapped to this region.


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