International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


UK MOUSE SEQUENCING PROGRAMME

AM Mallon
MRC UK Mouse Genome Centre and Mammalian Genetics Unit
Harwell
Oxfordshire OX11 0RD UK

Co-Authors: 1)P Denny, 2)MRM Botcherby, 4)P Gautier, 5)H Hummerich, 4)S Cross, 4)V van Heyningen, 2)N Leaves, 2)J Greystrong, 2)L Greenham, 2)S Jones, 2)K Maggott, 2)S Manjunath, 2)G Strachan, 3)M Strivens, 2)P North, 2)M Campell, 2)G Hunter, 2)G Kimberley, 2)L Cave-Berry, 3)L Mathews, 3)S Simms, 3)S Gregory, 3)R Evans, 3)T Hubbard, 3)R Durbin, 1)M Cadman, 1)R Mc Keone, 1)C Sellick, 5)M Iravani, 4)S White, 6)P Little, 4)I Jackson, 3)J Rogers, 2)RD Campbell, 1)SDM Brown
Institutions: 1) MRC UK Mouse Genome Centre and Mammalian Genetics Unit, 2) MRC UK-HGMP Resource Centre, 3) Sanger Centre, 4) MRC Human Genetics Unit, Western General Hospital, 5) MRC Prion Unit, St. Mary's Hospital Medical School, 6) School of Biochemistry & Molecular Genetics, University of New South Wales

The UK mouse sequencing programme commenced in October 1999 with the aim of sequencing 50 Mb of the mouse genome targeted to four core regions:

The WAGR-homologous region on mouse chromosome 2 The brown deletion complex on mouse chromosome 4 The Del(13)Svea36H chromosome 13 deletion Dmd-Ar region on chromosome X

For each of these four regions, there are already significant research efforts ongoing within the UK focused on the systematic determination of gene function through mutagenesis and other studies. The work of the consortium complements the MRC ENU mutagenesis programme, as three of its major target regions will be sequenced. In addition, 10% of the sequencing capacity is being devoted to additional requests by UK genomics researchers.

The programme will focus on the delivery of finished, contiguous sequence for these regions by 2002, improving the efficiency of mutation scanning and the identification of genes underlying mutations of interest. The discovery of candidate genes and features in these targeted regions is being undertaken by annotation generated from the Ensembl project (http://mouse.ensembl.org/) and also by cross-species comparison.

The mapping stage is nearing completion and has been carried out by the three mapping groups at HGU, Harwell and Imperial College. Significant draft and finished sequence is now available from all regions. A progress report on the sequencing will be presented along with an update on the analysis of sequence generated in the four core regions.


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