International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


MAPPING AND SEQUENCING THE MOUSE GENOME

John D McPherson
Genome Sequencing Center
Washington University School of Medicine
St. Louis MO 63108 USA

The mapping and sequencing capacity of the International Sequencing Consortium was greatly increased during the production of the working draft of the human genome. Until recently, this capacity has been utilized primarily for completing this genome. The finishing phase of the human genome will only occupy approximately 10% of this available capacity leaving large resources available to be applied to other genomes. A major focus of the Sanger, Washington University and Whitehead Institute genome sequencing centers is the generation of a working draft of the mouse genome as rapidly as possible and the completed sequence by 2005. To date, a BAC physical map has been generated by restriction digest fingerprinting and consists of 300,000 BAC clones ordered into less than 700 contigs. These contigs have been aligned to the mouse RH map and the human sequence map. Clones have been selected from these contigs for shotgun sequencing. To provide a working draft of the mouse genome in advance of the BAC-based sequence, a three-fold coverage of the genome has been generated in largely paired-ends reads. Plans are underway to supplement these sequences with two-fold more genome coverage using larger insert clones by the end of the year. The physical map and the assembled whole genome sequence reads will be a valuable tool for mouse research. Availability of these resources, progress to date and future plans will be presented.


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