International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 202 - SEQUENCING AND TRANSCRIPTION ANALYSIS OF THE Pdcd2 LOCUS ON MOUSE CHROMOSOME 17

Mr. Ondrej Mihola
Dept of Mammalian molecular genetics
Institute of molecular genetics
Videnska 1083
Prague
14220
Czech republic

Co-Authors: Trachtulec Zdenek, Forejt Jiri
Institutions: Institute of molecular genetics

The programmed cell death 2- (Pdcd2) gene was identified on the mouse chromosome 17 in the course of positional cloning of the mouse Hybrid sterility 1 locus. The Pdcd2 gene product consists of two domains: a MYND zinc-finger domain found in transcription factors and a highly conserved C- terminal domain of unknown function. Previously, the elevated transcription of the homologous rat Rp8 gene after triggering rat thymocytes to apoptosis was reported, but no such effect was found in the mouse.

The human homologous PDCD2 gene is alternatively transcribed, as judged from the matching ESTs. To investigate the alternative splicing of this gene in mouse, we sequenced the mouse Pdcd2 gene, including its flanking regions. The obtained sequence, 9.1 kb in length, was analysed by comparing it with the EST database and with the human PDCD2 gene. The constitutive mRNA of Pdcd2 gene consists of six exons. An alternative new exon of the mouse Pdcd2 gene was predicted and confirmed by RT-PCR. The exon replaces the last two constitutive exons, so that the alternative mRNA does not encode the highly conserved C-terminal domain. Moreover, an antisense mRNA of the alternative exon was identified. We hypothesize that the antisense transcript regulates the ratio of the constitutive and alternative mRNAs of Pdcd2 in the cell. Our finding of the alternative splicing of the Pdcd2 gene could explain the contradicting results from studies of apoptosis in rat and mouse.


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