International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 231 - REGIONAL RECESSIVE MUTAGENESIS SCREEN FOR CNS MUTATIONS: A 1ST YEAR REPORT

Darla Miller
Life Sciences Division
Oak Ridge National Laboratory
PO Box 2009
Oak Ridge
TN 37831-8077
USA

Co-Authors: Tennessee Mouse Genome Consortium
Institutions: Oak Ridge National Laboratory

The TMGC has initiated a regional ENU screen to generate recessive alleles that modulate CNS structure and function. Targets include Chrs 10, 15, 19, and X -roughly 15% of the genome. We use special tagged inversion stock and consomic strains  to generate pathogen-free test class animals . Our screens assess sensory-motor function (prepulse inhibition, tail suspension, exploratory activity), learning and memory using conditioned fear, qualitative and quantitative analysis of the CNS using 8 high throughput histological procedures, social behavior (interactions tests, response to odors, grooming), response to drugs of abuse (motor activity, novelty seeking, neophobias), alcohol response (EtOH-induced alterations in behavior and in EtOH preference), vision (eye morphology, retinal structure, optics), and aging (morbidity and mortality, behavioral and pathological changes with aging). Control strains include B6xC3HF2 and B6.PWK<19>. We have generated two lines of mice that have compromised viability and balance problems and typically die prior to weaning. One mutant has a marked neocortical abnormality while another has cerebellar abnormalities. This work is a component of a multicenter effort funded by 7 NIH institutes to accelerate functional genomic analysis of the mammalian CNS. Thanks to JL Guenet and MR Arnau Diaz Llanos for consomic stock). See www.tnmouse.org.


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