International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 204 - REPRESENTATIONAL DIFFERENCE ANALYSIS RESULTS IN THE IDENTIFICATION OF AN UNSTABLE REGION OF THE GENOME ON CHROMOSOME 11.

Dr. Bernard J. Morley
Imperial College
Rheumatology Section
Hammersmith Campus
Du Cane Road
London
W12 0NN
UK

Co-Authors: Fu G, Haywood MEK
Institutions: Rheumatology Section, Imperial College

BXSB mice develop a lupus-like autoimmune syndrome characterised by the production of autoantibodies to a spectrum of nuclear antigens.  In this study we have sought to isolate differentially expressed genes in an attempt to characterise lupus susceptibility genes within previously identified intervals.  Representational difference analysis (RDA) was used to compare gene expression between BXSB and C57BL/10 mice using spleen and thymus as a source of mRNA.  The majority of differentially expressed sequences identified were immunoglobulin and gp70-related sequences, overexpression of these being characteristic of the disease.  Among other isolated sequences were a sialyltransferase gene, a mouse tumour virus superantigen gene (Mtv-3), and the virus-related sequence, hitchhiker.  In BXSB the sialyltransferase gene not only overexpressed spliced transcripts, but also produced high levels of unspliced mRNA.  Further analysis demonstrated that the copy number of the three linked sequences: sialyltransferase, Mtv-3 and hitchhiker was amplified in BXSB and that the structural organisation of this locus varies in different mouse strains.  This locus consists of three parts, Mtv-3-hitchhiker-sialyltransferase, hitchhiker-sialyltransferase, and sialyltransferase alone.  Different combinations of the regions are present in different mouse strains.

 


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