International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


THE WHOLE GENOME ASSEMBLY OF MOUSE AND ITS IMPLICATIONS

Eugene W. Myers
Celera Genomics
45 West Gude Drive
Rockville Maryland 20850 USA

Co-Authors: Sutton G, Mural R, Li P, Yandell M, Halpern A, Smith H, Venter JC
Institution: Celera Genomics

The assembly of a shotgun data set of end-sequence reads was controversial in 1998 with critics claiming that it would require an impossibly large computation and result in a very fragmented and error-ladden assembly.

In 1999 the informatics research team at Celera produced an assembly of the 130Mbp Drosophila genome from a 13X whole genome shotgun data set, followed by an assembly of the Human genome in 2000 with a 5.1X data set and synthetic reads from public data. This year we produced an assembly of the Mouse genome from a 5.3X data set of 3 different mouse strains in equal proportions.

Our results from the mouse project prove unequivocally that whole genome shotgun sequencing is effective at delivering a highly reliable reconstruction with long-range order and orientation and dense polymorphism information. The fact that assembly is achieved at only 5.3X implies that a relatively complete picture of a large vertebrate genome can be obtained in six to nine months at very competitive cost with current technology. We demonstrate that the 5.3X assembly is a solid substrate for annotation and that it has great syntenic power when compared against the human genome.


Abstracts * Officers * Bylaws * Application Form * Meeting Calendar * Contact Information * Home * Resources * News and Views * Membership

Base url http://imgs.org
Last modified: Saturday, November 3, 2012