International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


POSTER 44 - GENE NET RECONSTRUCTION BASED ON MULTIPLE ANALYSIS OF DIALLEL CROSSES: GENETIC ARCHITECTURE OF HYPOTHALAMIC-PITUITARY-TESTICULAR AXIS IN MALE MICE

A.V. Osadchuk
Institute Cytology and Genetics of
Siberian Department of
Russian Academy of Sciences
630090 Novosibirsk
Russia

Co-Authors:. Svechnikov KV, Ahmerova LG, Kozlova ON 1) Huhtaniemi I
Institutions: Institute Cytology and Genetics of Siberian Department of Russian Academy of Sciences, 1) Department of Physiology, University of Turku

The aim of the study was to develop the simplest gene net model describing inheritable variation in the hypothalamic-pituitary-testicular axis in diallel crosses of six inbred mouse strains contrasting in plasma testosterone level, Leydig cells responsiveness (LCR), reproductive success and social dominance. We have performed a diallel analysis of plasma LH and testosterone levels, hypothalamic and hide brain noradrenaline contents, cAMP- and substrate-dependent testosterone productions by Leydig cells, androgen-dependent glands and body weights in mature males of six inbred strains (BALB/c, A/He, CBA/Lac, DD, YT and PT) and all their F1 hybrids. To detect the structure of genetic relationships between the traits, factor analysis of among crosses correlation matrix was conducted. It produced three factors that accounted for 61% of the total among crosses variance. They expressed the coordinated LCR, androgen status and gonadotrophic activity. Based on the multiple regression analysis, we developed an eight-locus gene net model accounting for 94% of the total variation due to these three factors. Four of these loci were linked to the sex chromosomes, the rest four did to the autosomes. Three of the autosomal loci manifested pleiotropic effects on the coordinated LCR and androgen status. One of them had also pleiotropic effects on gonadotrophic activity. Sex-linked loci manifested only factor-specific genetic effects. The gene net model included six digenic epistatic interactions between the gene net loci. In conclusion, the developed genetic model is a good tool for the molecular-genetic analysis of the hypothalamic-pituitary-testicular axis. (The work was supported by the grant RFFR 01-04-49523)


Abstracts * Officers * Bylaws * Application Form * Meeting Calendar * Contact Information * Home * Resources * News and Views * Membership

Base url http://imgs.org
Last modified: Saturday, November 3, 2012