International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)

POSTER 206 - Progress towards sequencing mouse Igh

Dr Roy Riblet
Torrey Pines Institute
3550 General Atomics Ct
San Diego
CA 92121

Our goal is to sequence the immunoglobulin heavy chain (Igh) locus of both BALB/c and C57BL/6 mice. These strains have been the primary models for studies of the antibody response and antibody genetics, and their Igh alleles (haplotypes) differ extensively. Complete Igh sequences from these strains will allow comparison of their germ line specificity repertoires (coding sequences) and will identify novel conserved noncoding regulatory elements for further study.

Initially, we assembled a 3 Mb YAC contig containing the C57BL/6 Igh locus, and we developed a detailed physical map of Igh. We have now assembled a BAC contig of Igh from C57BL/6 for complete sequencing as part of the NIH supported Mouse Genome Project. Sequencing of 18 BACs from this contig is in progress. Line 1 elements comprise an extraordinary 40-50% of the sequence of each BAC across the 3 Mb contig. Igh specific locally repetitive structure is common, and some BACs have virtually zero unique sequence.

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Last modified: Saturday, November 3, 2012