International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


A DNA MICROARRAY SCREEN FOR GENES FUNCTIONING IN MAMMALIAN SEXUAL DEVELOPMENT

Lee Smith
Medical Research Council
MRC Mammalian Genetics Unit
Harwell
Didcot OX11 0RD UK

Co-Authors: 1)Siggers P, 1)Van Hateren N, 1)Larder R, 1) Walsh J, 2) Stephens R, 2) Freeman T, 1)Greenfield A
Institutions: 1) MRC Mammalian Genetics Unit, 2) MRC HGMP Resource Centre

The mouse gonad and mesonephros originate from primordia that initially exhibit no morphological differences between males and females. The gonadal primordium (genital ridge) develops into a testis or ovary depending on the presence or absence of Sry in the gonadal supporting cell lineage between 10.5 and 13.0 days post coitum (dpc). The mesonephros also has a sexually dimorphic fate, depending on the presence or absence of hormonal signals from the testis. Several genes regulating these processes have been identified, but many gaps exist in these pathways.

We have used DNA microarrays to screen for genes exhibiting sexually dimorphic patterns of expression at different stages of mouse gonad and mesonephros development. These microarrays have been constructed using cDNA clones from a variety of embryonic sources. We present an overview of this work focussing on new genes whose patterns of expression suggest important roles in sexual development.


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