International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)


PPI NETWORK VIEWER: ANALYSIS OF 500 PROTEIN-PROTEIN INTERACTIONS OBTAINED FROM RIKEN MOUSE FULL-LENGTH cDNA

Harukazu Suzuki
RIKEN Genomic Sciences Center
1-7-22, Suehiro-cho, Tsurumi-ku
Yokohama 230-0045 Japan

Co-Authors: Saito R, Kanamori M, Miki R, Kagawa I, Bono H, Okazaki Y, Hayashizaki Y
Institutions: Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), Japan and Genome Science Laboratory, RIKEN Tsukuba Institute and Dept of Medicine, Tsukuba University

The RIKEN mouse encyclopedia project has already collected large number of mouse full-length enriched cDNAs, where many of them correspond to novel genes of unknown function. In order to uncover their function as a systematic genome-wide approach, we have developed a high-throughput protein-protein interaction (PPI) assay system based on a modified mammalian two-hybrid method (reported in the last IMGC meeting). We have now obtained >500 PPIs using 6,000 RIKEN cDNAs. For the detailed analysis of these PPIs, we have developed software, the PPI network viewer; we exhibited the strength of each interaction as a thickness of network line since the luciferase reporter activity roughly parallels the strength of interaction. This seems to be useful evaluating biological significance of the PPIs since two-hybrid data involve the pseudo-positive PPIs. Several intriguing PPIs that we found by the viewer will be reported. Further, we have made links from the viewer to our gene expression profiles and gene mapping data for the integrative analysis. As one of analyses, we have studied the correlation coefficient of gene expression profiles between the PPI pairs. The results show that the correlation between the pairs seems to depend on both the protein functions and the set of selected expression data. We will report the results and discuss how these analyses contribute to uncover the function of the novel genes.


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