International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)

POSTER 126 - ADAM   14-3-3   Cell Adhesion   Central Neural System

Dr. Pengcheng Zhu
Institute of Genetics, Fudan Univ.
Institute of Genetics,
Fudan University,
220# Handan Road, Shanghai, China

Co-Authors: R.Xu
Institutions: State Key Lab. of Genetic Engineering, Inst. of Genetics, Fudan University

Abstract ADAM family consists of a number of transmembrane proteins containing a disintegrin and metalloprotease domain. The ADAM proteins have both cell adhesion and protease activities and act in a highly diverse set of biological processes including fertilization, neurogenesis, myogenesis and inflammatory response. ADAM22 is highly expressed in the brain. The adam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. In present work, the protein 14-3-3zeta interacting with ADAM22 was detected by yeast two-hybrid assay. 14-3-3 is widely involved in many signal transduction pathways related to important processes of living cells, but this is the first time to demonstrate that 14-3-3 can interact with an ADAM family member. The specificity of interaction between ADAM22 and 14-3-3zeta was proved by binding assay and immunoprecipitation. We had also localized the major binding site of 14-3-3zeta in the last 28 amino acid residues of ADAM22 cytoplasmic tail. The interaction between ADAM22 and 14-3-3zeta suggests that the ADAM22 may play a crucial role in neural function and development.

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