International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 118 - CLONING OF A CANDIDATE GENE(S) FOR CALCUL RINSHOKEN (CaRin)

H. Yonekawa
The Tokyo Metropolitan Institute of Medical Science

1) Hirmoto A, 2) Kikkawa Y, 2)Taya C, 2) Ishii R, 2) Miura I, 1) Amano T, 3) Ishii Y, 3) Yoshikawa Y
1) Tokyo University of Agriculture, 2) The Tokyo Metropolitan Institute of Medical Science, 3) The University of Tokyo

A new mouse mutation, calacul Rinshoken (CaRin), arose in the transgenesis with human ?-galactosidase cDNA.  This mutation is autosomal dominant and exhibits waved coat hair and whiskers from the time of first appearance until about 4 weeks of age, and then the waves of coat hair and whiskers disappear, but the hair has a plush-like look. We mapped this mutation close to a classical wavy coat mutation, calacul (Ca), on Chr 15, which shows similar phenotype with our mutant.  We have constructed a genetic linkage map across the mutant locus in the intersubspecific backcross, [B6 x (B6-Ca/- x MSM)F1] (n=321).  In the backcross, CaRin was mapped to 12.5 cM distal toD15Mit14 and D15Mit77, and 2.5 cM proximal to D15Mit40 and ketatin gene complex-2, basic (Krt2).  Additionally, we have screened a YAC library and isolated one clone which includes D15Mit40 and Krt2. Then STS-markers were isolated from the clone.  Linkage analyses by those STS-markers confirmed that the CaRin locus extends about 800kb region in this YAC clone.  Krt2 genes are also localized in this region as a large cluster.  Eight expressed keratin genes were identified within the region, and their positions were more precisely located by a radiation hybrid (RH) panel. These results suggest that keratin genes are strong candidates for the wavy coat mutation.


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