International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 126 - AN ENU MUTAGENESIS SCREEN TO ISOLATE CARDIOVASCULAR AND HEMATOPOIETIC LETHAL MUTATIONS USING A MOUSE BALANCER CHROMOSOME

K Hentges
Baylor College of Medicine

2) Furuta Y, 1) Kaiser C, 2) Wang Y, 1) Lai L, 1) Hirschi KK, 1) Schwartz R, 2) Johnson RL, 3) Bradley A, 1) Justice MJ
1) Baylor College of Medicine, 2) University of Texas MD Anderson Cancer Center 3) Sanger Centre

Balancer chromosomes are a useful tool for mutation isolation, however, they have not previously been used in mice.  We used a Cre/loxP-engineered Chromosome (Chr) 11 balancer in a genetic screen designed to isolate recessive mouse mutations induced by N-ethyl-N-nitrosourea (ENU).  We have isolated 40 lines of mice on Chr 11 that are lethal before weaning. The time of death has been determined for several mutant lines, and ranges from pre-implantation to two weeks after birth.  An examination of mutant phenotypes reveals that many of the mutants are defective in the hematopoetic or cardiovascular system.  The phenotypes in these mutants include heart formation outside the amnion, internal hemorrhages late in gestation, and abnormal blood cell counts.  Markers of cardiac mesoderm, vascular endothelium, and blood cell development are being analyzed in mutants with phenotypes that suggest cardiovascular or hematopoietic defects.  Ongoing histological analysis of late gestation mutants will determine if the embryonic cardiovascular system is abnormal, causing the internal hemorrhage and eventual death of the embryo.  Continuing mutation isolation on this and other mouse chromosomes will identify novel members of pathways required for hematopoiesis and cardiovascular development.


Abstracts * Officers * Bylaws * Application Form * Meeting Calendar * Contact Information * Home * Resources * News and Views * Membership

Base url http://imgs.org
Last modified: Saturday, November 3, 2012