International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 135 - SCREENING MICE FOR NEUROLOGICAL MUTATIONS IN THE JAX-NEUROSCIENCE MUTAGENESIS FACILITY:  AN UPDATE

KL Seburn
The Jackson Laboratory

Frankel WN, JAX-NMF Staff
The Jackson Laboratory

The Jackson Laboratory Neuroscience Mutagenesis Facility (JAX-NMF) (http://nmf.jax.org/) is producing and making available mouse models of human CNS diseases using large-scale mutagenesis. The mutagen ENU is being used in genome-wide screens of C57BL/6J mice. Currently heritability has been confirmed for 11 potentially novel mutant lines in several different focus areas.  Four lines are fully established and mice are available for distribution with an additional 27 phenotypic deviants at various stages of heritability testing. High-throughput phenotype screens are underway in several focus areas including motor function, epilepsy, neural obesity, learning and memory, affective disorders, sensorimotor gating and vision.  Lower throughput screens for mice with mutations affecting ingestive behavior and smell are also underway and are being refined. Results vary from screen to screen, as some screens have only recently been implemented, but overall 25% of the families (equivalent to one mutagenized genome) tested to date had at least one phenotypic deviant in one of the screens. Heritability has been proven in 39% of the lines attempted.  A database that allows workflow management as well as storage and analysis of screening data has been constructed and refinements are ongoing.  Internet access to information related to all confirmed mutants is available.  Ongoing plans include the development of additional high-throughput screens in areas such as substance abuse, anxiety as well as expanded internet access to data generated by JAX-NMF via “live” links to our database.  Supported by NIH grant NS41215.


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