International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 163 - GENERATION OF HEPATOCELLULAR CARCINOMA IN THE C-H-RAS TRANSGENIC MICE IS VERY DIFFERENT DEPENDING ON THE MOUSE SEX

D.Y Yu
Korea Research Institute of Bioscience and Biotechnology

1) Wang A-G, 2) Moon H-B, 1) Yu S-L, 1) Lee D-S, 1) Lee T-H, 1) Yu D-Y
1) Korea Research Institute of Bioscience and Biotechnology, 2) School of Medicine, Wonkwang University

It was previously reported that the transgenic mice expressing c-H-ras mutant gene by the direction of albumin enhancer/promoter were born with enlarged livers and atypical hepatic architecture, and these all died within several days of birth (Oncogene 1989, 4, 715-724). In order to establish model mice generating hepatocellular carcinoma at a proper time for studying molecular event of hepatocellular carcinoma generation, we generated transgenic mice expressing c-H-ras mutant gene to the liver using albumin enhancer/promoter. We used c-H-ras cDNA mutated at 12 residue, Gly to Val, as a reporter gene.  2 lines of transgenic mice (28 and 76 lines) were generated.  The transgene was expressed at a very low level only in liver in the mice of 28 line and in all the tissues examined in the mice of 76 line. Grossly defined hepatocellular carcinomas were observed in all the mice of 28 line at the age of 6 months and appeared at the age of 9 months in 76 line mice.  Interesting finding is that male mice generated hepatic tumors several months earlier than female mice, suggesting that sex hormone might regulate hepatic tumor generation.  We are now continuing to know the reason why male mice generated hepatic tumors earlier than female mice.


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