International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 176 - A COMPLETED MOUSE GENETIC MAP: THE COMPREHENSIVE  T31 RADIATION HYBRID MAP CROSS REFERENCED AT LOD > 6 TO THE RECOMBINATION MAP FORMS A BASIS FOR SUPPORT OF THE SEQUENCE ASSEMBLIES

LB Rowe
The Jackson Laboratory

Barter ME, Kelmenson JA, Eppig JT
The Jackson Laboratory

We have collated the mouse T31 Radiation Hybrid (RH) data from all public sources into a single comprehensive map. As of Fall 2002 The Jackson Laboratory T31 mouse RH database (http://www.jax.org/resources/documents/cmdata/rhmap/RHIntro.html) contained about 4,000 distinct microsatellite anchor markers typed by several independent laboratories, over 17,000 unique expressed sequence tag (EST) loci mapped by three international genome centers, and hundreds of other loci. To date approximately one third of the mapped ESTs have identified gene functions. About 400 different laboratories worldwide have contributed RH data to this effort.To maximize the level of confidence for the mouse map and its usefulness in supporting the whole genome sequence assemblies, we have built a complete set of chromosome framework maps of known-order anchor markers between the RH and The Jackson Laboratory backcross panels. This recombination-based map contains about 6,000 loci mapped in a 188 animal interspecific backcross that has been in use by the community for the past nine years. The complete raw data and maps from this cross are available in a public database from http://www.jax.org/resources/documents/cmdata. The frameworking project has resulted in the correction of some genomic regions that were intially misordered in the RH data, the identification of mismapping D_Mit_ anchor loci, the detection of some data errors and omissions in the recombination map, and provides a high density of cross-links between the recombination and radiation hybrid maps that facilitate accurate genome-wide map construction. Future work will include direct order and distance correlation between this map and the mouse genome sequence.


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