International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


Oral Presentation

Sunday 17 November

16:00 - 16:15 HRS

MRC UK MOUSE SEQUENCING PROGRAMME – DETAILED ANALYSIS OF THE DEL(13)SVEA36H REGION ON MOUSE CHROMOSOME 13

JM Hancock
MRC UK Mouse Genome Centre and Mammalian Genetics Unit

Co-Authors: 1) Mallon AM, 1) Weekes J, 1) Hancock JM, 1) Denny P, 2) Ashurst J, 2) Wilming L, 2) Peyrefiwte S, 3) Botcherby MRM, 4) Gautier P, 5) Hummerich H, 4) Cross S, 4) van Heyningen V, 4) Edgar R, 3) Leaves N, 3) Greystrong J, 3) Greenham L, 3) Jones S, 3) Maggott K, 3) Manjunath S, 3) Russell E, 3) Strachan G, 1) Strivens M, 3) North P, 3) Boal E, 3) Cobley V, 3) Hunter G, 3) Kimberley G, 3) Cave-Berry L, 2) Mathews L, 2) Simms S, 2) Gregory S, 2) Evans R, 2) Hubbard T, 2) Durbin R, 1) Cadman M, 1) McKeone R, 1) Southwell A, 1) Sellick C, 5) Iravani M, 4) White S, 6) Little P, 4) Jackson I, 2) Rogers J, 3) Campbell RD & 1) Brown SDM
Institutions: 1) MRC UK Mouse Genome Centre and Mammalian Genetics Unit 2) Wellcome Trust Sanger Institute, 3) MRC UK-HGMP Resource Centre, 4) MRC Human Genetics Unit, 5) Imperial College, 6) School of Biochemistry & Molecular Genetics, University of New South Wales

The MRC UK Mouse sequencing programme has adopted a regional and functional approach, focussing on the delivery of finished, contiguous sequence for four substantial regions on chromosomes 2, 4, 13 and X.  The detailed manual annotation of this finished genomic sequence will improve the efficiency of mutation scanning and the identification of genes underlying mutations of interest within these regions. The region of mouse chromosome 13 being sequenced is encompassed by the Chromosome 13 deletion Del(13)Svea36H (hereafter referred to as Del36H).  This region was chosen for sequencing for a number of reasons but in particular because it is a target of an ENU mutagenesis programme underway at Harwell. This programme is using the deletion to uncover recessive phenotypes resulting from point mutations generated across the Del36H region.  The Del36H region spans approximately 14Mb of genomic DNA and is encompassed by a minimal tiling path of 95 BAC clones, with over 90% of these having finished genomic sequence to date. A combination of detailed manual and automated annotation is being performed across the region. Annotation of the sequence produced to date has demonstrated that the region is relatively gene dense, containing a number of gene clusters e.g. of histone and olfactory receptor genes. The region shows conserved synteny with human chromosome 6p22-21.3 and 6p25. We will present our annotation and comparative analysis of the Del36H region and a comparison of the finished sequence with the MGSC draft sequence assembly.


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