International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


Oral Presentation

Wednesday 20 November

10:00 - 10:15 HRS

MOLECULAR MAPPING OF THE MOUSE BRAIN

M. de Chaldée
CEA Saclay

Co-Authors: 1) Gaillard MC, 2) Bizat N, 1) Aude JC, 1) Buhler JM, 3) Manzoni O, 3) Bockaert J, 2) Hantraye P, 2) Brouillet E, 1) Elalouf JM
Institutions: 1) Service de Biochimie et de Génétique Moléculaire, Département de Biologie Joliot-Curie, CEA Saclay, 2) Service Hospitalier Frédéric Joliot, Département de Recherche Médicale, 3) CNRS UPR 9023

Transcriptome analysis of mouse brain territories is a potentially powerful approach to the diversity of mammalian cerebral functions. Here, we used a microadaptation (PNAS 96: 15286-15291) of the serial analysis of gene expression (SAGE) method to generate quantitative mRNA expression profiles of adult mouse whole brain, striatum, nucleus accumbens, and somatosensory cortex. A total of 118,622 cDNA tags were sequenced, corresponding to 35,732 different transcripts. Comparison of SAGE profiles led to the identification of 126 genes predominantly expressed in one brain region. Of these, two thirds remain without known function in the cerebral structure where they were detected. Their expression pattern was assessed within the brain and in peripheral organs using a combination of in situ hybridization, quantitative RT-PCR and Northern blotting. This approach has already allowed the validation of more than 20 new brain regional markers. Furthermore, several transcripts previously known as region-specific brain markers were detected. As an illustration, a number of expected striatal markers of different expression levels were found, including neuropeptides (preprotachykinin A, proenkephalin), receptors (dopamine receptor D2, striatum-specific G-protein coupled receptor GPR88), and proteins involved in signal transduction (e.g. adenylyl cyclase V, calmodulin-dependent phosphodiesterase PDE1B1, dopamine and cAMP-regulated phosphoprotein DARPP-32, protein tyrosine phosphatase STEP61, regulator of G-protein signaling Rgs9-2). However, most of the markers detected are novel, and thus provide a new insight into the physiology of the brain structures investigated.


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