International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


D Bogani
Mammalian Genetics Unit - Medical Research Council UK

Davies J, Alexander E, Heines H, Madge J, Napper H, Robertson L, McKeone R, Southwell A, Denny P, Arkell R
Mouse Genome Centre & Mammalian Genetics Unit, MRC

A recessive, two generation, screen for mouse mutants that affect postimplantation embryonic development is underway.  ENU induced mutations are recovered in the region of the Del(13)Svea36H (Del36H) deletion on chromosome 13. This cytogenetically visible deletion removes a 13 Mb region of MMu13 that includes the satin locus (Foxq1).  Mice that are homozygous null for Foxq1 have a glossy coat and are distinguishable from their littermates at 10 days post birth.  This locus is used to follow the mutagenised chromosome throughout the recessive screen.  In the screening protocol, male mice with a satin coat are mutagenised and crossed to wild type BALB/C females.  F1 male progeny are then crossed to female carriers of Del36H and the prevalence of progeny with a satin coat used to indicate those lines that potentially carry a new recessive lethal mutation.  Once the presence of a mutation is confirmed, the time of lethality and the associated embryonic phenotype is investigated, and recombination mapping is performed to further localise the mutation within the deleted region.  In parallel with this mutagenesis experiment, a BAC contig across the entire deleted region has been constructed and finished sequence of this contig has been generated and annotated (see abstract by Mallon et al).  The ongoing genomic analysis of this region has facilitated the mapping and cloning of the isolated mutants.  Results from the screen and the analysis of mutant phenotypes will be presented.

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