International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 36 - GENETIC AND BIOCHEMICAL ANALYSIS OF A NEW PUTATIVE E3 LIGASE

G Gao
ICMB

Wu J, Artzt K
ICMB, University of Texas at Austin, U.S.A.

A mutant first identified in zebrafish, mind bomb 1 (mib1), is currently being analyzed with reference to another vertebrate, the mouse.  This gene encodes a novel protein of ~ 1000 amino acids.  The protein sequence indicates a putative E3 ligase with three RING fingers.  Other domains include a ZZ-type Zinc finger flanked by two novel WXW domains and eight ankyrin repeats.  By searching ESTs in the database, a second homologue, mib2 has been identified.  Both mib1 and mib2 are conserved in Drosophila, zebrafish and mammals.  The mouse mib2 is 54% similar to mouse mib1 and 89% homologous to human mib2.  In zebrafish, mib1 is a recessive lethal with a known  serious disorder of the entire neuroepithelium tissue.  Thus, it would be interesting to determine the functions of mib2 and characterize any phenotype in the mouse.mib2 is located on mouse chromosome 4 at 79.4 cM and in human at 1p36.33.  The Mib2 protein is ubiquitously expressed in mouse.  Using RT-PCR, two alternative splice isoforms of mib2 have been cloned.  The first, mib2-1, encodes a protein with two RING fingers whereas the second, mib2-2, encodes a protein deficient of RING fingers.  The genetic and biochemical aspects of mib2 are being studied and to date, the protein has been purified and a knockout mouse is being generated by homologous recombination.


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