International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 6 - CONSTRUCTION OF INTERSPECIFIC CHROMOSOME SUBSTITUTION STRAINS

J Forejt
Institute of Molecular Genetics and Center for Integrated Genomics, Academy of Sciences of the Czech Republic

1) Aixnerova R, 1) Gregorova S,  1) Divina P, 2) Schalkwyk L
1) Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, and Center for Integrated Genomics, 2) Social, Genetic and Developmental Psychiatry Research Centre, Institute of Psychiatry

Various genetically defined mouse models have been devised to study quantitative traits, one of the most recent additions being the chromosome substitution strains (CSS). We have started construction of a panel of C57BL/6J-ChrPWD CSSs by transferring individual chromosomes from PWD/Ph, a Mus m. musculus - derived inbred strain, into the C57BL/6J recipient strain, predominantly of Mus m. domesticus origin. The rationale of using two species of mice was to exploit the extremely high degree of DNA polymorphism between both genomes that have been separated for the last 1 million years. Our preliminary estimate, based on the comparison of 80 RPCI23 BAC ends with corresponding PWD/Ph sequences, indicates the frequency of 1SNP per 339 bp. The progress in construction of the panel of 21 interspecies CSSs is following: The strains with Chromosomes 5, 7, 8, 13, 15, and Y are at BC5, Chromosomes 1, 9, 17,18 and X are at BC6, Chromosomes 4, 10, 11, 16 and 19 are at BC7 and Chromosomes 2, 3, 6, 12 and 14 are at BC9 generation. The average frequency of nonrecombinant PWD/Ph chromosomes is 0.215 among 2982 animals genotyped with 294 markers. The introgression of Chromosome X causes a male-specific hybrid sterility. Currently, we are mapping the genes on the X chromosome responsible for the infertility of male hybrids. Supported by EU grant QLRI-2000-00233 and by HHMI International Fellowship to J.F


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