International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 69 - GENE CATALOG OF MOUSE STEM CELLS AND EARLY EMBRYOS

M Ko
Developmental Genomics & Aging Section, Laboratory of Genetics; National Institute on Aging, NIH

1)Piao Y, 1) Stagg C, 1)Martin P, 1)Bassey U, 1)Qian Y, 1)Sharov A, 1)VanBuren V, 1)Tanaka T, 1)Jaradat S, 1)Carter M, 1)Kimber W, 1)Aiba K, 1)Hamatani T, 1)Yoshikawa T, 1)Matoba R, 1)Sharova L, 2)Kelso J, 2)Hide W
1) NIA, 2) South African National Bioinformatics Institute

We report here our continued efforts to assemble a mouse gene catalog from early embryonic stages as well as embryonic and adult stem cells. To overcome the scarcity of materials in these stages, we recently developed a method to construct long-insert enriched cDNA libraries from submicrogram amounts of total RNA. This new method allowed us to add ~80,000 new ESTs, which may represent full-length cDNAs, to our previous collection of ~80,000 ESTs. These ESTs were clustered into ~26,000 unique gene collections, which added ~11,000 new cDNA clones to the NIA Mouse 15K cDNA clone set. After single-colony isolation and sequence verification, a new 7.4K clone set was assembled and freely distributed to the research community. These ESTs and cDNA clone collections will provide essential tools to study early mammalian development and  the fundamental features of pluripotent stem cells.NIA Mouse cDNA Project Home Page: http://lgsun.grc.nia.nih.gov/cDNA/cDNA.html


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