International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)


POSTER 98 - TOWARDS FINE MAPPING TRYPANOSOMOSIS RESISTANCE QTL TIR2 AND 3 USING F12 TIR1 DEPLETED ADVANCED INTERCROSS LINE POPULATION

J Nganga
International Livestock Research Institute and Jomo Kenyatta University of Agriculture and Technology

1) Gibson, 2) Imbuga M J. 3) Kemp S, 1) Iraqi F
1) International Livestock Research Institute 2) Jomo Kenyatta University of Agriculture and Technology 3) School of Biological Sciences, University of Liverpool

Trypanosomosis resistance (Trypanotolerance) is exhibited by different inbred strains of mice after. T. congolense challenge. In a previous study, trypanotolerance quantitative trait loci (QTL) was mapped to chromosomes 17, 5 and 1, using two F2 resource populations (C57BL/6 x A/J and C57BL/6 x BALB/c), and designated as Tir1, Tir2 and Tir3 respectively. Using two F6 advanced intercross line (AIL) populations, Tir1 was mapped to less than 1cM, while Tir2 and 3, were mapped to large genomic intervals. In order to improve the resolution of Tir2 and 3 and identify possible candidate genes, F12 C57BL/6J x A/J AIL fixed for the susceptible and resistance alleles at Tir1 locus were generated. The two AIL populations homozygous for the resistant and susceptible Tir1 alleles together with the parental controls were challenged with T. congolense and followed for survival times over 180 days. Mice from the two survival extremes of the selected populations were genotyped with a panel of microsatellite markers across the previously mapped region and the data analysed with the web based QTL Express, Mapmaker/Exp and Mapmaker/QTL programs. QTL analysis showed that chromosome 1 comprise of three significant loci with their confidence interval being 3-6cM, while chromosome 5 had 1 locus with a 95% CI of 1cM. A number of candidate genes within these loci were identified based on their known function and possible association with response to trypanosomosis. Further investigations on expression and or sequence variation are now underway in order to a certain the role of this genes in trypanotolerance.


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